Immunomodulatory Effects of Astragalus gypsicolus Hydroalcoholic

Several studies have demonstrated that herbal extracts possess various biological effects

including anti-inflammatory and anti-cancer activities. The present study was aimed to

investigate the protective effects of the

early allergic sensitized mice induced by ovalbumin.

Phytochemical assay was used to recognize the main active constituents in the AG

hydroalcoholic extract. Mice were immunized with subcutaneous injection of ovalbumin and

aluminum hydroxide. Efficiency of sensitization was assessed by serum IgE levels and

eosinophil count. After sensitization, two doses of extract (250 mg/kg and 500 mg/kg) were

injected intrapritoneally.

On day 14, mice were challenged with intrapritoneal injection of ovalbumin. IL-4 and

IFN2 levels in broncoalveolar lavage fluid, which had been collected on day 15, were

assessed by Enzyme-Linked Immunosorbent Assay (ELISA) kit.

Our results indicate two main active constituents including flavonoids and terpenoids are

present in the AG hydroalcoholic extract. Intrapritoneal injection of the AG hydroalcoholic

extract was able to decrease IL-4 and increase IFN2. It seems the AG hydroalcoholic extract

has the potential to modulate the balance of Th1/Th2 cytokines in allergy.

Astragalus gypsicolus (AG) hydroalcoholic extract in